pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

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联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA) 
出品公司: 爱迪基因
目录编号: 115487
存储实验室: Steven Jacobsen

载体骨架基本信息
载体名称: pEG302
载体出品公司: --
载体抗性: 卡那霉素
空载体大小: --
完整质粒大小: --
载体修饰: --
载体类型: 植物表达,CRISPR,合成生物学
筛选标记: 潮霉素
高拷贝/低拷贝: --
生长条件: 37 ℃

插入基因信息
插入基因名称: g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS,22aa SunTag NtDRMcd g4+g10+g18
物种来源: --
插入大小: 17412 bp
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: --
克隆方法: --
5' 克隆位点: --
3' 克隆位点: --
5' 测序引物 --
3' 测序引物 --

文献引用方法
材料和方法部分: pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA) was a gift from Steven Jacobsen (Addgene plasmid # 115487 ; http://n2t.net/addgene:115487 ; RRID:Addgene_115487)
参考文献部分: Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722

载体图谱质粒图谱和多克隆位点信息

pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)质粒图谱

载体简介

载体序列

LOCUS       pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)               27445 bp ds-DNA     circular SYN 02-APR-2019
DEFINITION  CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with 
            three guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 27445)
  AUTHORS   Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
  TITLE     Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 
            SunTag systems.
  JOURNAL   Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
  PUBMED    30760722
REFERENCE   2  (bases 1 to 27445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 2, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..27445
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    4616..4640
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4659..4714
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             4728..8831
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8835..8861
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8880..8900
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8907..8927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8937..8957
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9021..9077
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9144..9200
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9267..9323
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9390..9446
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9513..9569
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9636..9692
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9759..9815
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9882..9938
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10005..10061
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10128..10184
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     terminator      10283..10990
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(11023..11041)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11024..11041)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11065..11082)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11065..11081)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11074..11096)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    11284..11308
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12609..13238
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13667..14740
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14806..15000
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15236..15258)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15344..15484
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15399..15418
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15499..15516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15670..16258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15750..15769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17683..18477)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    18902..18926
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    19016..19190
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(19231..20256)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(20323..20999)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(20366..20386)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        complement(21084..21159)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(21641..21716)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        complement(22203..22278)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(23029..23079)
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     CDS             complement(23107..23268)
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             complement(23299..23319)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(24439..25149)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(24479..24499)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     25094..25122
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(25240..25266)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agaagatgca gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac
       61 cacaacaccg tcgggacaga cgagaaacat gggaagctcc accacaatct tgggttaagt
      121 gcaactttga tggggcatgg ccaacagaag gattaaaatg tggcttaggg tgggtgcttc
      181 gcgatcatac agggaaggtg ttatggttag gtgcaagagc tgtggtaaaa gtaagaagcg
      241 tgctggaagt agaagtggag gctcttagat gggctgtgct gtcattatcc cgattcaatt
      301 ataggaagat catttttgag gtggattctc agcaacttgt gtctttggtt acatgaaagt
      361 tatgcttgtc aagtctcaat ccaattatcc aagacataaa gtatctactt agcaagtttg
      421 aggattttat gcttgtgcat acaagccgag aaggaaatgg agtggcagat agaatagcta
      481 aggaatctct ttcttttgag aattatgatc taaagttgta ttctattgta ccaatttggg
      541 ttaaaagctc tgttgagcta gactcatatg catccctagg aaacaagttg taatgagttg
      601 ctggcctctc ttgggattgt tcatcaaagt agctgtccct acacaccaca acagaatggt
      661 gttgtggaga gaaagcacag acacatcctt gagatggcaa gggcacttaa gtttcagagt
      721 ggtgtaccta ccaggttttg gggagactgt gtcaagactg ttgtatacct gatcaataga
      781 ctccctactc caatcttaca aggcaaatgt ccatatgaac tactctatca gaaacatgcc
      841 aagcttgatc acttgagagt gtttgggtgt cttgcttttg cagcaacact gcccaaaggt
      901 gacaaacttg cacctagagc caagcgaacc atcttcattg gttattctga gactcaaaag
      961 ggttacaggt tgtatgactt ggataataag gtgatcatag tgagcaggga tgtagttttc
     1021 agagagtttc agttcccttt caaagagtcc atctcccatg aacctgatat gttcactcga
     1081 gcaggttcac ttagctctga agatacagct ttgcagctag tgtataatga tatttttcct
     1141 tccagctttc atactgatga tgatgatact ccagttctta ttcctgacat tgtagaggcc
     1201 aatgaggata tcactttggg gactcattct acccaaaatc atatcacttc agctgatgct
     1261 gcaccttcag gccacctgca tgctcctgca gagtcagttg agtcagctga gcctgaccta
     1321 gcaaatgctg aatttgagca ccatactgct gtagctgatc cctctctagt tccagcaaac
     1381 ctgcacacta gacctaaacg caatgcaggt cctcccatct ggctcaagga ctttgtgaca
     1441 ctgaacaaag gctctaggga tgttccatac cctatagcca actatgtttc ctatgatcac
     1501 ttgcctgttc actatcaagc ttatttgagt gctttctcca cttatactga acctaaatcc
     1561 ttcaaagaag cagctcaaga tgagaaatgg atggaggcca tgtcccttga gatacaggct
     1621 cttgaggata ataacacctg ggagattgtc cctttacccc ctggtaaaca gcctataggg
     1681 tccaaatggg tgtacaaaat taaatacaaa gctaatggtg aagttgacag gtttaaggca
     1741 aggctagtgg ccaagggata cactcagcaa gaaggccttg actaccatga aactttttct
     1801 ccagtggcca aaatggtcac tgtaaaagct gtcatatctg ttgctgcttc caagggctgg
     1861 ttccttttcc agatggatgt caacaatgct tttctacaag gtgacctcat ggaagaagtt
     1921 tatatgtctc tgcctcaggg ttttcacagc caaggggaga ccaaagtgtg caggctgttg
     1981 aatccctcta tggtttaaag caagcatcaa ggcagtggaa catcaagctt accactgtcc
     2041 ttatgcaggc tggttttatg caaagtgctt atgatcactc cttgtttacc aaaagagagg
     2101 ggactgacct tgtcataatc ctgatttatg tggatgatct gttaataaca ggcagcagca
     2161 atgttttgat ttcagaagca aaggcaaccc tgcatcagca tttcaagatg aaagatttgg
     2221 gagaactaaa atacttctta ggcattgagg tgctgagatc agaaaaggga atcctactga
     2281 accagaggaa gtatgcactg gaactgatat caggtgttgg tctgggaggc tgcagaccag
     2341 tgtcaacacc aatggaacag aaccaaaggc tgactactgt agaatatgac aagcacttag
     2401 gaaagactga tgatgcagag ttagaggatg tgggttccta tcagagactg gtgggaaagc
     2461 ttctctactt gacaatcaca aggccagata tcagctttgc agtgcaggtg ctgagtcaat
     2521 tcatgcagca acccaaacag tcacacctgg aagcagcatt gagggttgtg aagtacataa
     2581 aaggttctcc aggtgttagg aacgcggccg catgcatatg agtctagctc aacagagctt
     2641 ttaacccaaa ttggtacaat agaatacaac tttagatcat aattctcaaa agaaagagat
     2701 tccttagcta ttctatctgc cactccattt ccttctcggc ttgtatgcac aagcataaaa
     2761 tcctcaaact tgctaagtag atactttatg tcttggataa ttggattgag acttgacaag
     2821 cataactttc atgtaaccaa agacacaagt tgctgagaat ccacctcaaa aatgatcttc
     2881 ctataattga atcgggataa tgacagcaca gcccatctaa gagcctccac ttctacttcc
     2941 agcacgcttc ttacttttac cacagctctt gcacctaacc ataacacctt ccctgtatga
     3001 tcgcgaagca cccaccctaa gccacatttt aatccttctg ttggccatgc cccatcaaag
     3061 ttgcacttaa cccaagattg tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt
     3121 tgtggttggt gctttcctta cattctgagc ctctttcctt ctaatccact catctgcatc
     3181 ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa gcaccagctc
     3241 gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca cattcttcag
     3301 atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa ctgatgatct
     3361 aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt tgtgtatcat
     3421 tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa cacgagtgtt
     3481 aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa caagaataaa
     3541 tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt gatacaaaag
     3601 tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta aaaaattaaa
     3661 agaaatggat aatttcacaa tatgttatac gataaagaag ttacttttcc aagaaattca
     3721 ctgattttat aagcccactt gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa
     3781 ataaagcacg aagaattcta gaaaatacga aatacgcttc aatgcagtgg gacccacggt
     3841 tcaattattg ccaattttca gctccaccgt atatttaaaa aataaaacga taatgctaaa
     3901 aaaatataaa tcgtaacgat cgttaaatct caacggctgg atcttatgac gaccgttaga
     3961 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
     4021 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
     4081 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
     4141 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
     4201 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
     4261 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
     4321 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
     4381 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
     4441 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
     4501 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
     4561 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagggat catcaacaag
     4621 tttgtacaaa aaagcaggct ctttaaagta tttttacaac aattaccaac aacaacaaac
     4681 aacaaacaac attacaatta ctatttacaa ttacaaaaaa agttaacatg gacaagaagt
     4741 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     4801 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     4861 agaacctgat cggcgccctg ctgttcgaca gcggagaaac agccgaggcc acccggctga
     4921 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     4981 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     5041 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     5101 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     5161 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     5221 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     5281 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     5341 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     5401 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
     5461 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     5521 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     5581 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     5641 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     5701 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     5761 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg
     5821 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     5881 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     5941 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     6001 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     6061 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     6121 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     6181 gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     6241 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga
     6301 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     6361 aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     6421 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     6481 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     6541 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
     6601 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
     6661 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
     6721 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
     6781 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
     6841 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
     6901 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
     6961 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
     7021 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
     7081 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
     7141 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
     7201 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga
     7261 gctttctgaa ggacgactcc atcgataaca aagtgctgac tcggagcgac aagaaccggg
     7321 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc
     7381 agctgctgaa tgccaagctg attacccaga ggaagttcga caatctgacc aaggccgaga
     7441 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
     7501 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     7561 agaacgacaa actgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     7621 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     7681 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     7741 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     7801 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     7861 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     7921 caaacggcga aacaggcgag atcgtgtggg ataagggccg ggactttgcc accgtgcgga
     7981 aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     8041 tcagcaaaga gtctatcctg cccaagagga acagcgacaa gctgatcgcc agaaagaagg
     8101 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     8161 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     8221 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     8281 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     8341 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
     8401 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     8461 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaaacact
     8521 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     8581 ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct atcagagagc
     8641 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     8701 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     8761 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     8821 tgggaggcga cgcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc
     8881 ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggaa gatgctccaa
     8941 agaagaagag aaaggtcgac ggcattggta gtgggagcaa cggcagcagc ggatccaacg
     9001 gtccgactga cgccgcggaa gaagaacttt tgagcaagaa ttatcatctt gagaacgaag
     9061 tggctcgtct taagaaaggt tctggcagtg gaggttctgg ctccggatct ggtggttcgg
     9121 gctcaggcgg gtccggatca ggcgaagaac tgctttcaaa gaattaccac ctggaaaatg
     9181 aggtagctag actgaaaaag gggagcggaa gtgggggctc cgggtcgggc tcagggggct
     9241 ccggttcggg aggctcaggg tcgggggagg agttgctgag caaaaattat catttggaga
     9301 acgaagtagc acgactaaag aaagggtccg gatcgggtgg ttcaggatct ggatccggag
     9361 gatcagggtc cggtgggtcg ggctcaggag aggagttact ctcgaaaaat tatcatctcg
     9421 aaaacgaagt ggctcggcta aaaaagggca gtggttctgg aggatctggg tcggggtcag
     9481 gcgggtctgg atctggggga tctggatctg gtgaagagct attatctaaa aactaccacc
     9541 tcgaaaatga ggtggcacgc ttaaaaaagg gaagtggcag tggtgggtcg ggatctggct
     9601 ctggtggctc aggctcggga ggttcaggtt ccggggaaga gctactatcc aagaattatc
     9661 atcttgagaa cgaggtagcg cgtttgaaga agggttccgg ctcaggagga tctgggtcag
     9721 gatcgggggg ttccgggtca ggcgggtccg ggtcaggcga ggaactgctc tcgaagaact
     9781 atcatcttga aaatgaggtc gctcgattaa aaaagggatc gggcagtggt gggtccggct
     9841 ccggttccgg aggatcggga tctgggggct cgggatccgg ggaggaacta ctttcaaaga
     9901 attaccacct cgaaaacgaa gtagctcgat taaagaaagg ttcagggtcg ggtggctcag
     9961 gttcgggatc aggtgggtca ggctccggtg gttcaggttc gggagaagaa ttactgagta
    10021 aaaattatca tctggaaaat gaggtagcga gactaaaaaa ggggagtggt tctggcggtt
    10081 cgggatctgg ctctgggggc tctgggtcgg gagggtctgg gtctggcgag gaattgctat
    10141 cgaaaaatta tcatcttgag aacgaagttg ctaggctcaa aaagggctca ggctcaggcg
    10201 ggtccgggtc agggtcggga ggttccggat ccgggggatc aggctcaggg taacccgggg
    10261 gcgcgccatg ataactagag tcctgcttta atgagatatg cgagacgcct atgatcgcat
    10321 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga
    10381 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt
    10441 atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt
    10501 aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca
    10561 caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc
    10621 ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg
    10681 ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt
    10741 ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg
    10801 aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc
    10861 ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac
    10921 cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga
    10981 ggctcagcag gaccggcatg caagctagct tactagtgat attctatagt gtcacctaaa
    11041 tctctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
    11101 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    11161 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    11221 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
    11281 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
    11341 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
    11401 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
    11461 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
    11521 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
    11581 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
    11641 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
    11701 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
    11761 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
    11821 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
    11881 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
    11941 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
    12001 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
    12061 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
    12121 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
    12181 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
    12241 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
    12301 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
    12361 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
    12421 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
    12481 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
    12541 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
    12601 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
    12661 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
    12721 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
    12781 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
    12841 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
    12901 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
    12961 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
    13021 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
    13081 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
    13141 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
    13201 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
    13261 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
    13321 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
    13381 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
    13441 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
    13501 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
    13561 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
    13621 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
    13681 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
    13741 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
    13801 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
    13861 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
    13921 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
    13981 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
    14041 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
    14101 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
    14161 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
    14221 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
    14281 gcgtacaaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
    14341 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
    14401 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
    14461 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
    14521 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
    14581 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
    14641 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    14701 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    14761 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
    14821 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    14881 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    14941 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    15001 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    15061 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    15121 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    15181 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    15241 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    15301 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
    15361 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
    15421 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    15481 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    15541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    15601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    15661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    15721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    15781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    15841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    15901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    15961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    16021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    16081 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    16141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    16201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    16261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    16321 gctgatgaat cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt
    16381 gtcatatgat caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc
    16441 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
    16501 agcttaacgt tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt
    16561 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
    16621 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
    16681 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa
    16741 aaacggctta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
    16801 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
    16861 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
    16921 aaagtgccat ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga
    16981 ggaaaagtac aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat
    17041 gatatgtcat ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca
    17101 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
    17161 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
    17221 tggattctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
    17281 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
    17341 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
    17401 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
    17461 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
    17521 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct
    17581 gcaaccctac tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg
    17641 gatctctcag cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
    17701 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    17761 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    17821 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    17881 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    17941 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    18001 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    18061 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    18121 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    18181 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    18241 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    18301 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    18361 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    18421 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    18481 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    18541 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    18601 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    18661 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    18721 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    18781 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    18841 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    18901 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
    18961 ggacgttttt aatgtactga attaacgccg aattaattcg agctctaatt cgggggatct
    19021 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
    19081 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
    19141 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa ctcgagcttg
    19201 tcgatcgaca gatcccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
    19261 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
    19321 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
    19381 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
    19441 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
    19501 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
    19561 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
    19621 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
    19681 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
    19741 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
    19801 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
    19861 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg
    19921 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgaacggcg
    19981 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg
    20041 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
    20101 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
    20161 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
    20221 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cggatctgcg
    20281 aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
    20341 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
    20401 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
    20461 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    20521 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
    20581 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
    20641 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
    20701 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca
    20761 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    20821 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    20881 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    20941 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    21001 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    21061 gctccaccat ggtaccaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
    21121 actagcctta ttttaacttg ctatttctag ctctaaaacc tctttatccc attcaacatc
    21181 aatcactact tcgactctag ctgtatataa actcagcttc gttttcttat ctaagcgatg
    21241 tgggactttt gaagattgtt ttcaacttaa atgggcctat ataagaaata ctattgttct
    21301 ttcccatata aatgggcctg cttctcttct ttcagattcc caggggcctt ttgaagatta
    21361 tcttcatatc ttaagaatga agatgtttta ttcaatcaaa ttcttgaagg ttcgatgcct
    21421 aatcattcta atcctgggac aaactatgaa acaagataca aaaactccga atggaaagtt
    21481 aaaaagaaga aaacgaaagc tacggttcaa gaaaatgtaa gctgataaac aaaaaaaaac
    21541 tgtatgaacg aagaagaaga aaaaaagcta agaagaaatg atgtattgtg cggaaggcaa
    21601 gtcgagtttc cgttgttcaa cgaaagcttg tttaaaaaaa gcaccgactc ggtgccactt
    21661 tttcaagttg ataacggact agccttattt taacttgcta tttctagctc taaaacttca
    21721 acattcatac gagcgccaat cactacttcg actctagctg tatataaact cagcttcgtt
    21781 ttcttatcta agcgatgtgg gacttttgaa gattgttttc aacttaaatg ggcctatata
    21841 agaaatacta ttgttctttc ccatataaat gggcctgctt ctcttctttc agattcccag
    21901 gggccttttg aagattatct tcatatctta agaatgaaga tgttttattc aatcaaattc
    21961 ttgaaggttc gatgcctaat cattctaatc ctgggacaaa ctatgaaaca agatacaaaa
    22021 actccgaatg gaaagttaaa aagaagaaaa cgaaagctac ggttcaagaa aatgtaagct
    22081 gataaacaaa aaaaaactgt atgaacgaag aagaagaaaa aaagctaaga agaaatgatg
    22141 tattgtgcgg aaggcaagtc gagtttccgt tgttcaacga aagctttcct aaaccaaaaa
    22201 aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc
    22261 tatttctagc tctaaaacaa gcccatacat ctttccgtca atcactactt cgactctagc
    22321 tgtatataaa ctcagcttcg ttttcttatc taagcgatgt gggacttttg aagattgttt
    22381 tcaacttaaa tgggcctata taagaaatac tattgttctt tcccatataa atgggcctgc
    22441 ttctcttctt tcagattccc aggggccttt tgaagattat cttcatatct taagaatgaa
    22501 gatgttttat tcaatcaaat tcttgaaggt tcgatgccta atcattctaa tcctgggaca
    22561 aactatgaaa caagatacaa aaactccgaa tggaaagtta aaaagaagaa aacgaaagct
    22621 acggttcaag aaaatgtaag ctgataaaca aaaaaaaact gtatgaacga agaagaagaa
    22681 aaaaagctaa gaagaaatga tgtattgtgc ggaaggcaag tcgagtttcc gttgttcaac
    22741 gaaagcttcg taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
    22801 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
    22861 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
    22921 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
    22981 aaatgtttga acgatcgggg aaattcgagc tcaaacttac ggccatggcg taggcggcct
    23041 tttccgttgt gatcttcgcg gtctcctcct tgtttttgga ctagtaccac caccgctacc
    23101 accaccttcg gttaccgtga aggttttggt agcgtcgtcg taggtccatt caccgtcaac
    23161 accgttgtcg ttagcgtact gtttgaaaac tttttccgcg gtagcagcgt caacagcttc
    23221 ggtggtggtt tcacctttca gggttttacc gttcaggata agcttgtact cttcggtccg
    23281 agatcctcct cccgtacgga cctttctctt cttctttgga gcccctccgc caccgccatg
    23341 tttatgtctg gacattatgg acttgaccaa gtccaatatc cgaacatagt cataaaatag
    23401 ggaagactct ttgccttcaa gcccatccct gctcactctg ttactgcctg caaggttatt
    23461 gcacgggctt ccaccaatta ccaaatcaaa tcctccaaat gactctatca gttgctccaa
    23521 gcggtctcca ttcagctgct gcacatcgtt aaaatgtata aggttccctc tctgattagt
    23581 ttgctcccac cagcttctca caatatccct gttgacttca gatttttcca cagaaaccac
    23641 tgtgtttagt ggaataccaa gacgataaag agcaacttca ccaccgccaa ttccagagaa
    23701 gagtgataag acattgatac caccgggaaa caagtctttc aataccgaca aatggtatgc
    23761 cacagtgtca acctggaatg aattaccaag cgacttgtat ctatcggtcc tacttatacc
    23821 acctccccta gtatggttct ttggaaatcc cagtagcatt tcaacttcat caggctccaa
    23881 aggagcaact ttgtttctcc caacccacac caaattccac ttccgacatt gatcaagaac
    23941 aaacttctga actctcattg gtggctcacc atcaaagtcc tccacagctt tcctaatcct
    24001 attcgtcaat tgtgcacttc caatggctgt ttgcaaacaa tttaactttg tccgtggatc
    24061 ccaagatggc caccatttct tcgatagggg aagtgcctca tgaatggtac gtggggcaag
    24121 tggaaacaaa ggaaatctat tttcaaccgg cagattatga atataacccc tttttcttgc
    24181 agcagcacaa aaatatttgg agtcgacaaa ctctggctca atatcgtaca aaaatctaga
    24241 aattgtgtcc cacacaccct ttggagctag agccacattt tcatagtaga aaaacggggg
    24301 tccaacagct tgctcgggaa gagttcttcg aaccattgct ggaagtggtt cggtaggaac
    24361 cccaaaccca atcataggtt tgggcaaacg aattgtctcc cctccgccac cgcccgtacg
    24421 accggtccga cctccacctt tgtagagctc atccatgcca tgtgtaatcc cagcagcagt
    24481 tacaaactca agaaggacca tgtggtcacg cttttcgttg ggatctttcg aaaggacaga
    24541 ttgtgtcgac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt
    24601 ttgttgataa tggtctgcta gttgaacgga accatcttca acgttgtggc gaattttgaa
    24661 gttagctttg attccattct tttgtttgtc tgccgtgatg tatacattgt gtgagttaaa
    24721 gttgtactcg agtttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccctttaa
    24781 ctcgatacga ttaacaaggg tatcaccttc aaacttgact tcagcacgcg tcttgtaggt
    24841 cccgtcatct ttgaaagata tagtgcgttc ctgtacataa ccttcgggca tggcactctt
    24901 gaaaaagtca tgccgtttca tgtgatccgg ataacgggaa aagcattgaa caccataggt
    24961 cagagtagtg acaagtgttg gccacggaac aggtagtttt ccagtagtgc aaataaattt
    25021 aagggtgagt tttccgtttg tagcatcacc ttcaccctct ccacggacag aaaatttgtg
    25081 cccattaaca tcaccatcta attcaacaag aattgggaca actccagtga aaagttcttc
    25141 tcctttgcta ccgcttccac ctccacctgg atccaagctt ccgccgcctc ctgaaccacc
    25201 accgccacta cctcctcccc cagaacctcc gcctccacca gcgtaatctg gaacatcgta
    25261 tgggtagctg ctcacggtca ccagggtgcc ctggccccag tagtcgaaca ggccggtcac
    25321 gcagtagtac agggcggtgt cgtcgctgcg caccttgctc atctgcaggt acacggtgtt
    25381 cttgccgttg tccttgctga tgatgaagcg gtccttcagg gcgctgttgt agtcggtgat
    25441 gccgtcgccc cagatcacgc cgatccactc caggccgcgg ccgggggcct ggcgcaccca
    25501 gttcacgccg tagtcggtca ggctgaagcc gctcacggcg cagctcagct tcaggctgcc
    25561 gccgggctgc accaggccgc cgccgctctc cagcagcttc acctcgctgc cgccgccgct
    25621 gctgccgccg ccgccgctgc cgccgccgcc gctgccgccg ccgccgcgct tcagctccac
    25681 cttggtgccc tggccgaaca cccagtggtt gctgtaccac agggcgcaga agtaggtggc
    25741 gaagtcctcg ggctgcaggc tgctgatggt cagggtggcc ttgtcgccga tcaggctgcc
    25801 gctgaagcgg ctgggcacgc cgggggcgcg gttgttggtg ccgccgatca ggcccttgaa
    25861 cagcttgccg ggcttctcct gcacccagct ggcgtagttg ctggtggtca cggcgccggt
    25921 gctgctgcgg caggtgatgg tcacgcggtc gcccacgctg gcgctcaggc tgctggggct
    25981 ctgggtcatc acgatgtcgg ggcccatcag cgctaaaccc taggtgttaa tcagaaaaac
    26041 tcagattaat cgacaaattc gatcgcacaa actagaaact aacaccagat ctagatagaa
    26101 atcacaaatc gaagagtaat tattcgacaa aactcaaatt atttgaacaa atcggatgat
    26161 atctatgaaa ccctaatcga gaattaagat gatatctaac gatcaaaccc agaaaatcgt
    26221 cttcgatcta agattaacag aatctaaacc aaagaacata tacgaaattg ggatcgaacg
    26281 aaaacaaaat cgaagatttt gagagaataa ggaacacaga aatttacctt gatcacggta
    26341 gagagaattg agagaaagtt tttaagattt tgagaaattg aaatctgaat tgtgaagaag
    26401 aagagctctt tgggtattgt tttatagaag aagaagaaga aaagacgagg acgactaggt
    26461 cacgagaaag ctaaggcggt gaagcaatag ctaataataa aatgacacgt gtattgagcg
    26521 ttgtttacac gcaaagttgt ttttggctaa ttgccttatt tttaggttga ggaaaagtat
    26581 ttgtgctttg agttgataaa cacgactcgt gtgtgccggc tgcaaccact ttgacgccgt
    26641 ttattactga ctcgtcgaca accacaattt ctaacggtcg tcataagatc cagccgttga
    26701 gatttaacga tcgttacgat ttatattttt ttagcattat cgttttattt tttaaatata
    26761 cggtggagct gaaaattggc aataattgaa ccgtgggtcc cactgcattg aagcgtattt
    26821 cgtattttct agaattcttc gtgctttatt tcttttcctt tttgtttttt tttgccattt
    26881 atctaatgca agtgggctta taaaatcagt gaatttcttg gaaaagtaac ttctttatcg
    26941 tataacatat tgtgaaatta tccatttctt ttaatttttt agtgttattg gatatttttg
    27001 tatgattatt gatttgcata ggataatgac ttttgtatca agttggtgaa caagtctcgt
    27061 taaaaaaggc aagtggtttg gtgactcgat ttattcttgt tatttaattc atatatcaat
    27121 ggatcttatt tggggcctgg tccatattta acactcgtgt tcagtccaat gaccaataat
    27181 attttttcat taataacaat gtaacaagaa tgatacacaa aacattcttt gaataagttc
    27241 gctatgaaga agggaactta tccggtccta gatcatcagt tcatacaaac ctccatagag
    27301 ttcaacatct taaacaagaa tatcctgatc tgaagaatgt ggaggcttta gtcccttgga
    27361 tacttgggag gctgtggaag aacagaaacg agctggtgct taaagggagg gaatttggaa
    27421 ccaatgaggt attagtaagg acaca
//

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